Learn how to safely extract lymph nodes from deer and elk for Chronic Wasting Disease (CWD) testing. This detailed tutorial covers identification, handling, and proper sample packaging for submission to CDFW.
In this informative guide, Brandon Monk, a wildlife veterinarian for the California Department of Fish and Wildlife, provides a step-by-step tutorial on how to remove lymph nodes from deer and elk for Chronic Wasting Disease (CWD) testing. Chronic Wasting Disease poses a significant threat to deer and elk populations, as well as hunting opportunities. This tutorial aims to help hunters and wildlife enthusiasts ensure the health of these species by demonstrating the correct procedure for extracting lymph nodes.
Identification of Key Landmarks To begin the process, you need to locate specific anatomical landmarks on the deer or elk. These landmarks include the jaw bone, trachea, and larynx. The trachea's widest part marks the location of the larynx, which is where you'll make your initial incision.
Making the Incision With the deer or elk positioned on its back and its head and neck extended, a sharp knife is used to make a precise incision. The initial cut should go straight through the trachea, followed by inserting an index finger into the windpipe to facilitate further cutting toward the base of the ears.
Locating the Lymph Nodes The retropharyngeal lymph nodes, which are the primary target of this procedure, are situated on either side of the midline. These lymph nodes should feel like discrete, semi-firm structures that can be gently pinched and pulled.
Extraction Techniques There are two methods for removing the lymph nodes. The first involves gently dissecting the tissue around the lymph nodes with your fingers until you can grasp and remove them. The alternative method uses a knife and tweezers to cut them out.
Characteristics of Lymph Nodes Lymph nodes vary in size, shape, and color between animals but are generally kidney-shaped, approximately nickel to quarter-sized, and tan to light red. To confirm that you've extracted a lymph node, bisect the sample along its short axis to reveal discrete, lighter-colored areas around the outside edge, which are the lymphoid follicles.
Packaging for Submission The tutorial also covers how to properly package the lymph nodes for submission to the California Department of Fish and Wildlife. Each lymph node should be placed in a sample bag, which is then sealed, squeezing out excess air. The data card, including your GOID and tag number, must also be completed and inserted into the specimen bag.
Storage and Submission Depending on when you plan to submit the samples, they can be refrigerated or frozen. The video outlines the options for dropping off the samples at a CDFW regional office and provides a website for additional sampling and drop-off options. The results of the CWD testing can also be found on the website.
Patience in Testing It's essential to be patient after submitting your samples, as CWD testing may take a few months to complete. The video concludes with contact information for any additional questions or inquiries.
This tutorial serves as a valuable resource for hunters and wildlife enthusiasts looking to contribute to the monitoring and management of CWD in deer and elk populations, helping to ensure the continued health and sustainability of these animals.
For further information and updates, please visit wildlife.ca.gov/CWD.
Remember, early detection and responsible management are crucial in combating Chronic Wasting Disease, safeguarding both the wildlife populations and hunting opportunities.
All rights reserved. Unauthorized reproduction, in whole or in part is strictly prohibited.
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